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Universal primers and PCR of gut contents to study marine invertebrate diets
Blankenship, L.E.; Yayanos, A.A. (2005). Universal primers and PCR of gut contents to study marine invertebrate diets. Mol. Ecol. 14(3): 1-9
In: Molecular Ecology. Blackwell: Oxford. ISSN 0962-1083, more
Peer reviewed article  

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    Cytochrome-c oxidase; Cytochromes; Deep water; Diatoms; Diets; DNA; Food organisms; Gutting; Identification; Marine crustaceans; Marine invertebrates; Marine molluscs; Mitochondria; Nucleotide sequence; Phylogeny; Polymerase chain reaction; Predation; Primers; Stomach content; Amphipoda [WoRMS]; Bacillariophyceae [WoRMS]; Bivalvia [WoRMS]; Eurythenes gryllus (Lichtenstein in Mandt, 1822) [WoRMS]; Lucinoma aequizonata (Stearns, 1890) [WoRMS]; Lucinoma Dall, 1901 [WoRMS]; Scopelocheirus Bate, 1856 [WoRMS]; Scopelocheirus schellenbergi Birstein & Vinogradov, 1958 [WoRMS]; Marine

Authors  Top 
  • Blankenship, L.E.
  • Yayanos, A.A.

    Determining the diets of marine invertebrates by gut content analysis is problematic. Many consumed organisms become unrecognizable once partly digested, while those with hard remains (e.g. diatom skeletons) may bias the analysis. Here, we adapt DNA-based methods similar to those used for microbial diversity surveys as a novel approach to study the diets of macrophagous (the deep-sea amphipods Scopelocheirus schellenbergi and Eurythenes gryllus) and microphagous (the bivalve Lucinoma aequizonata) feeders in the deep sea. Polymerase chain reaction (PCR) in conjunction with 'universal' primers amplified portions of the mitochondrial cytochrome c oxidase I (COI) gene for animals ingested by S. Schellenbergi and E. Gryllus and the 18S rRNA gene for lesser eukaryotes ingested by L. Aequizonata. Amplified sequences were combined with sequences from GenBank to construct phylogenetic trees of ingested organisms. Our analyses indicate that S. schellenbergi, E. Gryllus and L. Aequizonata diets are considerably more diverse than previously thought, casting new light on the foraging strategies of these species. Finally, we discuss the strengths and weaknesses of this technique and its potential applicability to diet analyses of other invertebrates.

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