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Universal primers and PCR of gut contents to study marine invertebrate diets
Blankenship, L.E.; Yayanos, A.A. (2005). Universal primers and PCR of gut contents to study marine invertebrate diets. Mol. Ecol. 14(3): 891-899. https://dx.doi.org/10.1111/j.1365-294X.2005.02448.x
In: Molecular Ecology. Blackwell: Oxford. ISSN 0962-1083; e-ISSN 1365-294X, more
Peer reviewed article  

Available in  Authors 

Keywords
    Acids > Organic compounds > Organic acids > Nucleic acids > DNA
    Algae > Diatoms
    Aquatic organisms > Food organisms
    Aquatic organisms > Marine organisms > Aquatic animals > Marine invertebrates
    Biogeny > Phylogeny
    Cell constituents > Cell organelles > Mitochondria
    Diets
    Enzymes > Coenzymes > Cytochromes
    Fauna > Aquatic organisms > Aquatic animals > Shellfish > Marine organisms > Marine crustaceans
    Fauna > Aquatic organisms > Aquatic animals > Shellfish > Marine organisms > Marine molluscs
    Handling > Fish handling > Dressing > Gutting
    Identification
    Interspecific relationships > Predation
    Materials > Coating materials > Primers
    Nucleotide sequence
    Peptides > Proteins > Enzymes > Oxidoreductases > Cytochrome-c oxidase
    Polymerase chain reaction
    Stomach content
    Water > Deep water
    Amphipoda [WoRMS]; Bacillariophyceae [WoRMS]; Bivalvia [WoRMS]; Eurythenes gryllus (Lichtenstein in Mandt, 1822) [WoRMS]; Lucinoma aequizonata (Stearns, 1890) [WoRMS]; Lucinoma Dall, 1901 [WoRMS]; Scopelocheirus Spence Bate, 1857 [WoRMS]; Scopelocheirus schellenbergi Birstein & M. Vinogradov, 1958 [WoRMS]
    Marine/Coastal

Authors  Top 
  • Blankenship, L.E.
  • Yayanos, A.A.

Abstract
    Determining the diets of marine invertebrates by gut content analysis is problematic. Many consumed organisms become unrecognizable once partly digested, while those with hard remains (e.g. diatom skeletons) may bias the analysis. Here, we adapt DNA-based methods similar to those used for microbial diversity surveys as a novel approach to study the diets of macrophagous (the deep-sea amphipods Scopelocheirus schellenbergi and Eurythenes gryllus) and microphagous (the bivalve Lucinoma aequizonata) feeders in the deep sea. Polymerase chain reaction (PCR) in conjunction with 'universal' primers amplified portions of the mitochondrial cytochrome c oxidase I (COI) gene for animals ingested by S. Schellenbergi and E. Gryllus and the 18S rRNA gene for lesser eukaryotes ingested by L. Aequizonata. Amplified sequences were combined with sequences from GenBank to construct phylogenetic trees of ingested organisms. Our analyses indicate that S. schellenbergi, E. Gryllus and L. Aequizonata diets are considerably more diverse than previously thought, casting new light on the foraging strategies of these species. Finally, we discuss the strengths and weaknesses of this technique and its potential applicability to diet analyses of other invertebrates.

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