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| The changes in digestive enzymes and hormones of gilthead seabream larvae (Sparus aurata, L 1758) fed on Artemia nauplii enriched with free methionine |  |
| Naz, M.; Türkmen, M. (2009). The changes in digestive enzymes and hormones of gilthead seabream larvae (Sparus aurata, L 1758) fed on Artemia nauplii enriched with free methionine Aquacult. Int. 17(3): 243-256 |
| In: Aquaculture International. Chapman & Hall/Kluwer/Springer: London. ISSN 0967-6120, meer |
| Trefwoorden |
Alkaline phosphatase; Amylase; Bombesin; Cholecystokinin; Trypsin; Trypsin; Sparus aurata Linnaeus, 1758 [Goudbrasem] [WoRMS]; Marien |
| Abstract |
Variations in digestive enzymes and hormones during the larval development
of gilthead seabream (Sparus aurata) fed on live prey (Artemia nauplii) enriched with free
methionine were investigated for 16 days (from day 24 to day 40). Prior to initiation of the
experiment, newly hatched larvae were transferred from incubators to fiber glass tanks
(300 l) with black walls and fed with same diets until day 24. Each experiment was
performed in triplicate. In the experimental group, the content of the free methionine in the
Artemia nauplii was increased by adding a 5.3 mM free methionine solution to the culture
water during a 16-h enrichment period. The larvae of both the control and enrichedmethionine
groups were sampled four times, with 4-day intervals between samplings,
during a 16-day period. The larvae in the control group had a significantly lower growth
than those of the methionine group at the end of the study (P>0.05). The highest trypsin
activity and leucine aminopeptidase N/leucine–alanine peptidase ratios were observed in
the control group. A significant difference between bombesin activities in the treatment
groups was not found at 5th minute after the initiation of feeding (P>0.05), but they were
significant at 15th minute post-initiation of feeding (P>0.05). A significant difference
between the cholecystokinin levels of the treatment groups was found (P>0.05). |
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