||Open Marine Archive
|Standardization of protocol for Vibrio challenge in specific pathogen-free (SPF) shrimp (Litopenaeus vannamei)|
|Le Hong, P.; Sorgeloos, P.; Bossier, P. (2007). Standardization of protocol for Vibrio challenge in specific pathogen-free (SPF) shrimp (Litopenaeus vannamei), in: Mees, J. et al. (Ed.) (2007). VLIZ Young Scientists' Day, Brugge, Belgium 2 March 2007: book of abstracts. VLIZ Special Publication, 39: pp. 39|
|In: Mees, J.; Seys, J. (Ed.) (2007). VLIZ Young Scientists' Day, Brugge, Belgium 2 March 2007: book of abstracts. VLIZ Special Publication, 39. Vlaams Instituut voor de Zee (VLIZ): Oostende. IX, 82 pp., meer|
|In: VLIZ Special Publication.. Vlaams Instituut voor de Zee (VLIZ): Oostende. ISSN 1377-0950, meer|
Aquaculture techniques; Garnaalkweek; Ziektekiemen; Litopenaeus vannamei [WoRMS]; Vibrio campbellii; Marien
This study was conducted to standardize the protocol for Vibrio challenge in specific pathogen-free (SPF) shrimp (Litopenaeus vannamei). Shrimp, from postlarvae15 to juvenile, were challenged with Vibrio campbellii, V. harveyi 642, V. harveyi E022, V. harveyi E2, and V. penaeicida by immersion and injection in normal as well as stress conditions. For immersion challenge, shrimp were immersed in seawater containing 103, 105, and 107 CFU mL-1 of bacterial cells. They were fed with Artemia nauplii, Artemia charged with V. campbellii, a piece of shrimp meat which was injected with the Vibrio campbellii or artificial feed during five days experiment. Bacterial density in the water and shrimp samples was determined at the end of experiment. There was no significant difference in mortality between the control and Vibrio challenged groups in normal conditions (p>0.05). Therefore, different stress factors were tested such as starvation, salinity and ammonium. 12h exposure to 40 and 50 mgL-1 NH4 + (pH = 7.9- 8.1) were found as sublethal doses for postlarvae and juvenile shrimps respectively. Starvation, ammonium stress (before and during challenge periods), and salinity stress at 5, 10, 20 gL-1 did not enhance the susceptibility of shrimp to Vibrio challenge by immersion. In absence of stress, SPF shrimp is not susceptible to Vibrio either by immersion or injection. Only in ammonium stress, SPF shrimp is susceptible to Vibrios to a large extent by injection with 106 CFU shrimp-1. Vibrio campbellii was found as the most virulent strain towards SPF shrimp among five tested strains.