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| Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia fransiscana |  |
| Nelis, H.J.; Merchie, G.; Lavens, P.; Sorgeloos, P.; De Leenheer, A.P. (1994). Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia fransiscana Anal. Chem. 66(8): 1330-1333 |
| In: Analytical chemistry: Washington. ISSN 0003-2700, meer |
| Ook gepubliceerd als |
- Nelis, H.J.; Merchie, G.; Lavens, P.; Sorgeloos, P.; De Leenheer, A.P. (1994). Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia fransiscana, in: (1994). IZWO Coll. Rep. 24(1994). IZWO Collected Reprints, 24: pp. chapter 1994 [Subsequent publication], meer
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| Trefwoorden |
Artemia franciscana; Marien; Brak water |
| Abstract |
Two producers are described for the solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia . The first one is based on reversed-phase ion pair extraction on an octadecylsilica cartridge using tetrabutylammonium as the counterion, followed by elution with methanol. In the second procedure ascorbic acid 2-sulfate is retained on a DEAE silica cartridge and eluted with 0.1M sodium salicylate. Both approaches were used as part of a quantitative reversed-phase ion pair liquid chromatographic method with detection at 254nm. The anion exchange extraction procedure is superior to its ion pair counterpart in terms of recovery (88.7±3,5% versus 63.0±12.7%), reproducibility (CVs of 3.6-7.7% versus 6.3-18.7%), and simplicity. |
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