| one publication added to basket [59087] | Genome size of Adriatic seagrasses
In: Aquatic Botany. Elsevier Science: Tokyo; Oxford; New York; London; Amsterdam. ISSN 0304-3770; e-ISSN 1879-1522, meer
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| Trefwoorden |
Acids > Organic compounds > Organic acids > Nucleic acids > DNA
Flora > Weeds > Marine organisms > Seaweeds > Sea grass
Genomes
Alismatidae; Cymodocea nodosa (Ucria) Ascherson, 1870 [WoRMS]; Pisum sativum; Posidonia oceanica (Linnaeus) Delile, 1813 [WoRMS]; Zostera subg. Zostera marina Linnaeus, 1753 [WoRMS]; Zostera subg. Zosterella noltei Hornemann [WoRMS]
MED, Adriatic [Marine Regions]
Marien/Kust |
| Author keywords |
seagrasses; Alismatidae; genome size; C-value; DNA image cytometry |
| Auteurs | | Top |
- Koce, J.D.
- Vilhar, B.
- Bohanec, B.
- Dermastia, M.
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| Abstract |
Genome size (C-value) was measured in four species of Adriatic seagrasses with interphase-peak DNA image cytometry. The estimated 2C-value was 1.5 pg DNA for Zostera noltii (2n=12), 1.2 pg for Zostera marina (2n=12), 1.1 pg for Cymodocea nodosa (2n=28) and 6.2 pg for Posidonia oceanica, using Pisum sativum (2C-value=8.84 pg) as the calibration standard. Seagrass leaves were fixed in 4% buffered formaldehyde to mitigate stoichiometric error due to tannins and post-fixed in 3:1 methanol:acetic acid (MAA). DNA was stained with the Feulgen reaction after hydrolysis in 5 M HCl for 90 min at 20 °C. Comparison of genome size of seagrasses with the data for other species of Alismatidae indicated that the ancestral genome of Alismatidae was relatively small. |
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