IMIS

Publicaties | Instituten | Personen | Datasets | Projecten | Kaarten
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp. ALG-5
Kime, D.E.; Lee, E.Y.; Kim, H.S. (2009). Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp. ALG-5. Mar. Biotechnol. 11(1): 10-16. https://dx.doi.org/10.1007/s10126-008-9114-9
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

Trefwoorden
    Oligosaccharides
    Products > Fishery products > Processed fishery products > Seaweed products > Alginates
    Marien/Kust
Author keywords
    Alginate lyase; Alginate oligosaccharide; Streptomyces sp ALG-5;Unsaturated uronate

Auteurs  Top 
  • Kime, D.E.
  • Lee, E.Y.
  • Kim, H.S.

Abstract
    A marine bacterium was isolated from seaweeds for its ability to degrade alginate. Analysis of 16S ribosomal DNA sequence and chemotaxonomic characterizations revealed that the strain belongs to Streptomyces. The alginate lyase gene of Streptomyces sp. ALG-5 was cloned by using PCR with the specific primer designed from homologous nucleotide sequences. The consensus sequences of N-terminal YXRSELREM and C-terminal YFKAGXYXQ were conserved in the ALG-5 alginate lyase gene. The recombinant alginate lyase was purified by using Ni-Sepharose affinity chromatography. The alginate lyase appears to be poly-guluronate lyase degrading poly-G block preferentially than poly-M block. The degraded products were determined to be di-, tri-, tetra- and pentasaccharides by using BioGel P-2 gel filtration chromatography and ionization mass spectroscopy method.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs