The use of high pressure liquid chromatography with fluorimetric detection for chlorophyll A determination in natural extracts of chloropigments and their degradation products
Goeyens, L.; Post, E.; Dehairst, F.; Vandenhoudt, A.; Baeyens, W.F.J. (1982). The use of high pressure liquid chromatography with fluorimetric detection for chlorophyll A determination in natural extracts of chloropigments and their degradation products. Int. J. Environ. Anal. Chem. 12: 51-63
In: International journal of environmental and analytical chemistry. Taylor & Francis: Amsterdam. ISSN 0306-7319; e-ISSN 1029-0397, meer
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Chromatography
Organic compounds > Carbohydrates > Glycosides > Pigments > Photosynthetic pigments > Chlorophylls
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| Auteurs | | Top |
- Goeyens, L., meer
- Post, E.
- Dehairst, F.
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- Vandenhoudt, A.
- Baeyens, W.F.J., meer
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| Abstract |
To realize chlorophyll A analysis, a method is proposed that combines the sensitivity of fluorimetric determinations and the inherent selectivity and accuracy of chromatographic separations. A partial application of the overlapping resolution mapping (ORM) method led to the use of a methanol:acetone:water (75:22:3, v/v) mixture for the isocratic separation by high pressure liquid chromatography on a reserved phase C-18 column of at least sixteen chloropigments and degradation products in a natural extract. Ten replicate measurements of a 50 µg.l-1 standard showed a reproducibility of 3 promille while a detection limit of 84 pg chlorophyll A was estimated. |
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