Vlaams Instituut voor de Zee

Platform voor marien onderzoek

In:

Open Marien Archief

Het Open Marien Archief van België (OMA) biedt vrije toegang tot de digitale publicaties over de Vlaamse kust en het Belgisch deel van de Noordzee, en alle andere mariene, estuariene en kustgebonden publicaties van Belgische auteurs en wetenschappers en van buitenlandse wetenschappers geaffilieerd aan een Belgische instelling.

Meer info

Nieuwe zoekopdracht
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print-vriendelijke versie

Metallothioneins and cytosolic metals in Neomysis integer exposed to cadmium at different salinities
Erk, M.; Muyssen, B.T.A.; Ghekiere, A.; Janssen, C.R. (2008). Metallothioneins and cytosolic metals in Neomysis integer exposed to cadmium at different salinities. Mar. Environ. Res. 65(5): 437-444. dx.doi.org/10.1016/j.marenvres.2008.02.002
In: Marine Environmental Research. Applied Science Publishers: Essex. ISSN 0141-1136, meer
Peer reviewed article

Beschikbaar in Auteurs 

Trefwoorden
    Bio-accumulatie; Cadmium; Marien milieu; Metabolisme; Metallothioneins; Osmosis; Saliniteit; Voltammetry; Zoutwater; Zware metalen; Arthropoda [WoRMS]; Crustacea [WoRMS]; Mysida [WoRMS]; Mysidacea [WoRMS]; Neomysis integer (Leach, 1814) [WoRMS]; Marien; Brak water
Author keywords
    mysid; Neomysis integer; cadmium; salinity; metallothionein; cytosolic metals

Auteurs  Top 

Abstract
    In the present study the induction of metallothioneins (MTs) and its relation to cytosolic metal concentrations (Zn, Cu and Cd) in the euryhaline crustacean Neomysis integer exposed to Cd at different salinities was studied. N. integer was exposed to the same free cadmium ion activity of 5.74 x — 10-9 mol l-1 (i.e. 1/5 of the 96 h LC50 value expressed as cadmium activity) in hypo-osmotic (5 psu), isosmotic (16 psu) and hyper-osmotic media (25 psu) for 7 days. In this way, the effect of salinity on cadmium speciation was eliminated and therefore the physiological effect of salinity on Cd accumulation and MT induction could be studied. The accumulation of cytosolic Cd in N. integer changed with salinity from 1.11 ± 0.05 µmol l -1 at 5 psu up to 1.43 ± 0.17 µmol l-1 at 25 psu. This could indicate that the physiological response of euryhaline estuarine invertebrates like N. integer to salinity changes can influence the rate of trace metal uptake from solution. While the salinity changes did not cause significant differences in cytosolic Zn concentrations (mean value of all tested salinities: 34.4 ± 2.8 µmol l-1), an inverse relationship between salinity and cytosolic Cu concentration was observed. The highest concentration of 15.7 ± 2.3 µmol Cu l-1 was determined at 5 psu and the lowest 10.9 ± 1.4 µmol Cu l-1 at 25 psu. This could point to a possible relationship between the copper concentration and the hemocyanin metabolism in N. integer. This is the first time that differential pulse voltammetry method was applied to MT assays with N. integer. Although the exposure to Cd resulted in a higher Cd cytosolic concentration, no subsequent MT increase was detected. The significant positive correlation between MT levels and cytosolic Cu concentrations (Spearman correlation coefficient rs = 0.356, p = 0.009) implies a strong relationship between MT and Cu in N. integer.

 Top | Auteurs