|Intercalibration exercise on the qualitative and quantitative analysis of fatty acids in Artemia and marine samples used in mariculture|
Coutteau, P.; Sorgeloos, P. (1995). Intercalibration exercise on the qualitative and quantitative analysis of fatty acids in Artemia and marine samples used in mariculture. ICES Coop. Res. Rep. 211: 1-30
In: ICES Cooperative Research Report. ICES: Copenhagen. ISSN 1017-6195, meer
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- Coutteau, P.; Sorgeloos, P. (1995). Intercalibration exercise on the qualitative and quantitative analysis of fatty acids in Artemia and marine samples used in mariculture, in: (1995). IZWO Coll. Rep. 25(1995). IZWO Collected Reprints, 25: pp. chapter 7, meer
Artemia Leach, 1819 [WoRMS]; Marien
An international intercalibration exercise was conducted to evaluate the accuracy of the ICES Standard Methodology for fatty acid analysis in a sample of Artemia and a formulated dry feed. Results were received from 11 of the 20 laboratories to which samples were sent. Five participants followed the Standard Method, five their own in-house method and one laboratory compared their own in-house method with the Standard Method. Total lipid content was reported by eight participants.
The average intra-laboratory variation in the determination of total lipid content was only 3.6% (CV) for the dry feed and 4.0% for the Artemia nauplii. The inter-laboratory variation was somewhat higher being 5.2% for the dry feed and 8.7% for nauplii. In addition, significant differences were found between lipid content reported by the different laboratories. Nevertheless, the inter-laboratory variation obtained in this study was considerably lower than that reported in a previous intercalibration exercise (Léger et al, 1989). It is suggested that this may have been because precise procedures were prescribed for both hatching the cysts and lipid extraction.
Intra- and inter-laboratory variability in the determination of fatty acid composition was on average twice as high for quantitative data as it was for qualitative data. The intra-laboratory variation, averaged for all the laboratories for the feed and Artemia respectively, was as low as 3.3% and 2.7% for qualitative data, and 6.9% and 6.3% for quantitative data. In comparison, the average inter-laboratory variation for the major fatty acids in the feed and Artemia respectively, was 13.7% and 7.3% for qualitative data, and 24.5% and 11.5% for quantitative data. The higher variability in the quantitative, as well as qualitative data, for the dry feed may have been due to a higher variability in the extraction and/or methylation of the fatty acids from the extruded matrix of the diet compared to brine shrimp tissue. The laboratories using the Standard Method exhibited a somewhat lower intra-laboratory and inter-laboratory variation for the qualitative values than the laboratories applying their own in-house method. In contrast, the quantitative analyses revealed, particularly for the dry feed, a slightly higher variability for the laboratories following Standard Method. The overall variability in the present exercise, both on the intra-laboratory as well as inter-laboratory level, was significantly lower than that reported by Léger et al. (1989). The better accuracy obtained in the present exercise for the determination of fatty acid composition in Artemia nauplii is at least partially due to the stipulation of a standard procedure for hatching and analysis of the cyst sample in the instruction to the participants. Although it is more elaborate and solvent consuming than many current methods for fatty acid analysis in routine use, the ICES Standard Method may be used to intercalibrate the analytical procedures adopted by different laboratories to analyse fatty acids in Artemia and marine samples.