The effect of para-nonylphenol on Japanese eel (Anguilla japonica) spermatogenesis in vitro
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514, more
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| Keywords |
Aromatics > Phenols
Biology > Physiology > Endocrinology
Cells > Sertoli cells
Morphogenesis > Gametogenesis > Spermatogenesis
Anguilla japonica Temminck & Schlegel, 1846 [WoRMS]
Marine/Coastal |
| Author keywords |
nonylphenol; Japanese eel; spermatogenesis; endocrine disruption;11-ketotestosterone; testis; sertoli cells; estradiol-17 beta |
| Authors | | Top |
- Miura, C.
- Takahashi, N.
- Michino, F.
- Miura, T.
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| Abstract |
Endocrine disrupters have been recognized to interfere with endocrine systems that regulate reproduction, for example, by mimicking or inhibiting the action of endogenous sex steroid hormones including estradiol-17β (E2). In the present study, we examined the effect of an endocrine disrupter, para-nonylphenol (p-NP) on spermatogenesis, and compared it with the action of E2, using an eel testicular organ culture system. p-NP alone stimulated early spermatogonial renewal in the same manner as E2. Neither induced further progress in spermatogenesis. In the presence of 11-ketotestosterone (11-KT), the major androgen in teleosts, p-NP did not prevent the 11-KT-induced progress in spermatogenesis. However, this treatment enlarged the Sertoli cells. Electron microscopic observation revealed that enlarged Sertoli cells contained well-developed organelles. Moreover, the proportion of germ cells appeared to have decreased as a result of Sertoli cell hypertrophy. These results clearly show that p-NP has an effect on Sertoli cells in the presence of an androgen (11-KT), potentially disturbing 11-KT-induced spermatogenesis. |
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