Preliminary study on linkage mapping based on microsatellite DNA and AFLP markers using homozygous clonal fish in ayu (Plecoglossus altivelis)
Watanabe, T.; Fujita, H.; Yamasaki, K.; Seki, S.; Taniguchi, N. (2004). Preliminary study on linkage mapping based on microsatellite DNA and AFLP markers using homozygous clonal fish in ayu (Plecoglossus altivelis). Mar. Biotechnol. 6(4): 327-334. http://dx.doi.org/10.1007/s10126-003-0026-4
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236, more
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| Keywords |
Acids > Organic compounds > Organic acids > Nucleic acids > DNA
Aflp
Fishes > Osteichthyes > Salmoniformes > Plecoglossidae > Plecoglossus > Freshwater fishes > Ayu
Plecoglossus altivelis (Temminck & Schlegel, 1846) [WoRMS]
ISEW, Japan [Marine Regions]
Marine/Coastal |
| Author keywords |
AFLPs; ayu (Plecoglossus altivelis); linkage map; microsatellite DNA |
| Authors | | Top |
- Watanabe, T.
- Fujita, H.
- Yamasaki, K.
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| Abstract |
A mapping referential family (F-1) of ayu was produced by crossing a normal diploid male with a homozygous clonal female. A genetic linkage map was constructed using 191 amplified fragment length polymorphism (AFLP) and 4 microsatellite DNA markers. A total of 178 loci were mapped in 36 linkage groups comprising 1659.6 cM, which includes approximately 77.3% to 81.8% of the total genome. As the markers were randomly distributed over the genome, they showed high efficiency for the construction of a wide linkage map. |
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