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GRL: GEANS Reference Library
Citation
Christodoulou; 2021; GEANS Reference Library

Availability: Unrestricted after moratorium period
Data are initially restricted, but the access condition relaxes to academic or unrestricted once a specified period of time after an event (such as collection, publication, completion of QC procedures or project cessation) has elapsed

Notes: until project end (31 August 2022)

Description
Benthic macroinvertebrates are key components in environmental impact assessments. Nevertheless, their use as bioindicators can be constrained by the time- and cost-consuming processes needed for their morphological identification. The recent advances in high-throughput sequencing, particularly DNA metabarcoding can provide an alternative to morphology-based approaches. The main limitation for DNA-based tools to be implemented in biomonitoring projects is the considerable investment needed to build high-quality and curated taxonomic reference DNA sequence libraries for species identification. To begin addressing this shortage, GEANS project aims in developing a curated DNA reference library based on mitochondrial cytochrome c oxidase subunit I (COI) for the North Sea macrobenthos that will serve as the backbone of all the molecular protocols. more

For the collection of the macroinvertebrates, targeted sampling campaigntake place across the North Sea Region, while existing collections are being scanned. Macrobenthic specimens are being processed following a series of standardise work flows covering sampling, morphological identifications, molecular lab processing and data handling. All specimens are photographed and together with the accompanying sequences, collection and taxonomic data are archived in a dedicate BOLD project. Voucher specimens and DNA extracts are archived at three different institutes in Germany (Senckenberg am Meer), the Netherlands (Naturalis) and Belgium (ILVO). Once available, this DNA-library will be based on taxonomically well-curated specimens and it will support the implementation of fast, cost-efficient and reliable DNA-based identifications and subsequently environmental health assessments in the North Sea.

Scope
Themes:
Biology, Biology > Benthos, Biology > Benthos > Macrobenthos
Keywords:
Marine, Brackish water, Biodiversity, COI, Dna, DNA barcoding, Genetic, Genetic data, ANE, North Sea

Geographical coverage
ANE, North Sea [Marine Regions]

Temporal coverage
1 March 2019 - 28 February 2022
Quasi continuous (< 1 min.)

Contributors
Naturalis Biodiversity Center (NBC), moredata creator
Senckenberg am Meer; German Centre for Marine Biodiversity Research (DZMB), more
Vlaamse overheid; Beleidsdomein Landbouw en Visserij; Instituut voor landbouw-, visserij en voedingsonderzoek (ILVO), moredata creator

Related datasets
Parent dataset:
GEANS Data: Genetic tools for Ecosystem health Assessment in the North Sea region, more

Project
GEANS: Genetic Tools for Ecosystem Health Assessment in the North Sea Region, more

Dataset status: In Progress
Data type: Data
Data origin: Data collection
Metadatarecord created: 2020-03-20
Information last updated: 2021-05-20
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy