Molecular phylogeny of mangroves: 9. Molecular marker assisted intra-specific variation and species relationships in the Indian mangrove tribe Rhizophoreae
Lakshmi, M.; Parani, M.; Parida, A. (2002). Molecular phylogeny of mangroves: 9. Molecular marker assisted intra-specific variation and species relationships in the Indian mangrove tribe Rhizophoreae. Aquat. Bot. 74(3): 201-217. http://dx.doi.org/10.1016/s0304-3770(02)00105-5
In: Aquatic Botany. Elsevier Science: Tokyo; Oxford; New York; London; Amsterdam. ISSN 0304-3770; e-ISSN 1879-1522, more
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| Keywords |
Acids > Organic compounds > Organic acids > Nucleic acids > DNA
Biogeny > Phylogeny
Biomarkers
Classification > Taxonomy > Chemotaxonomy
Conservation
Genetic diversity
Hybrids
Intraspecific relationships
Mangroves
Bruguiera cylindrica (L.) Blume [WoRMS]; Bruguiera gymnorrhiza (L.) Lam. [WoRMS]; Bruguiera parviflora (Roxb.) Wight & Arn. ex Griff. [WoRMS]; Ceriops decandra (Griff.) W.Theob. [WoRMS]; Ceriops tagal (Perr.) C.B. Robinson [WoRMS]; Rhizophora apiculata Blume [WoRMS]; Rhizophora mucronata Poir. [WoRMS]; Rhizophora stylosa Griff. [WoRMS]
ISW, India [Marine Regions]; ISW, India, Andaman I. [Marine Regions]; ISW, India, Nicobar I. [Marine Regions]
Brackish water |
| Author keywords |
Rhizophoraceae; mangrove; molecular markers; phylogeny; conservation |
| Authors | | Top |
- Lakshmi, M.
- Parani, M.
- Parida, A.
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| Abstract |
Molecular phylogeny and genetic diversity in all the nine species (Rhizophora mucronata, R. apiculata, R. stylosa, Bruguira cylindrical, B. parviflora,B. gymnorriza, Ceriops tagal and C. decandra) and a natural Rhizophora hybrid, of the mangrove Rhizophoraceae, occurring in the Indian sub-continent were analysed using random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) and RFLP of polymerase chain reaction amplified chloroplast genes (PCR-RFLP) as markers. Intra-specific variability as revealed by RAPDs were low in all the analysed species. Inter-specific RFLP analysis revealed species-specific profiles in some probe-enzyme combinations. The rDNA repeat units, as flanked by the Hind III restriction sites was found to be very conserved within each genus and three different rDNA repeat units were observed among the four genera. Generic differences in PCR-RFLP were observed only in rbcL and trnS-psbC gene regions. Species differences were observed in Rhizophora in the PCR-RFLP of trnS-psbC and trnL-UAA with Hae III and Taq I, respectively. Rhizophora mucronata was found to be the chloroplast donor for the natural inter-specific hybrid. A dendrogram based on the data sets from all the three marker systems revealed that the four genera segregated into three species groups. |
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