The development of neutral lipase and phospholipase activities was studied in larval turbot fed live prey. Activities of neutral lipase and phospholipase (activity larva-1) increased significantly between days 6 and 24 after hatching in turbot larvae. The specific activities of both enzymes (activity µg protein-1) decreased in older larvae. Feeding of a microdiet for 3 days (days 10-13) affected the lipolytic activity of neutral lipase and phospholipase negatively, compared to the larvae fed on rotifers. Since neutral lipase activities in whole larval homogenates and in the gut were significantly lower, it suggests a reduced synthesis rate and a reduced secretion of the enzyme in larvae fed the microdiet. A correlation between neutral lipase and phospholipase activities was found in larvae fed rotifers, but not in larvae fed the microdiet. This may indicate different regulating and stimulating mechanisms for these enzymes. The contribution of exogenous enzymes from ingested live prey to the total larval enzyme activity was about 6% for neutral lipase and 10% for phospholipase on day 6. The exogenous prey enzymes accounted for only 2% of the total activities in 12-day-old turbot larvae, suggesting that enzymes from prey did not contribute considerably to the digestion of lipids.