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Comparison of cytochemical procedures to estimate lysosomal biomarkers in mussel digestive cells
Marigómez, I.; Lekube, X.; Cajaraville, M.P.; Domouhtsidou, G.; Dimitriadis, V. (2005). Comparison of cytochemical procedures to estimate lysosomal biomarkers in mussel digestive cells. Aquat. Toxicol. 75(1): 86-95. https://dx.doi.org/10.1016/j.aquatox.2005.07.002
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514, more
Peer reviewed article  

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Keywords
    Biomarkers
    Cell constituents > Cell organelles > Lysosomes
    Eukaryotes > Animals > Invertebrates > Mollusca > Bivalvia > Shellfish > Mussels
    Mussels
    Pollution > Water pollution > Marine pollution
    Marine/Coastal
Author keywords
    lysosomal membrane stability; lysosomal structural changes; biomarkers;mussels; marine pollution

Authors  Top 
  • Marigómez, I.
  • Lekube, X.
  • Cajaraville, M.P.
  • Domouhtsidou, G.
  • Dimitriadis, V.

Abstract
    Enlargement and membrane destabilisation in digestive cell lysosomes of mussels are biomarkers of pollution effect. Cytochemical methods are currently applied to determine lysosomal membrane stability (LMS) and lysosomal structural changes (LSC). LMS, determined after grading N-acetyl-β-hexosaminidase activity on cryotome sections of digestive gland, is measured as labilisation period (LP). LSC, determined after image analysis of cryotome sections where β-glucuronidase activity is revealed, are measured as lysosomal volume (Vv), surface (Sv), numerical (Nv) densities and surface-to-volume ratio (S/V). Both methods have now been compared in a field study. Mussels were collected from Biscay Bay (Plentzia, reference; Muskiz, moderately polluted) and North Aegean Sea (Olympiada, reference; Limani, heavily polluted). Higher Vv and Sv and lower S/V and LP were recorded in polluted sites than in reference sites. Significant correlations with LP were found for Vv and S/V. The cost/effectiveness and environmental significance of both methods are discussed.

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