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GRL: GEANS Reference Library
Christodoulou M.; Beentjes K.; van der Hoorn B., Van Den Bulcke, L., Derycke S., De Backer, A.; Martinez Arbizu P.; 2021; GEANS Reference Library

Beschikbaarheid: Vrij beschikbaar na embargo periode
Data are initially restricted, but the access condition relaxes to academic or unrestricted once a specified period of time after an event (such as collection, publication, completion of QC procedures or project cessation) has elapsed

Nota: until project end (30 june 2023)

Benthic macroinvertebrates are key components in environmental impact assessments. Nevertheless, their use as bioindicators can be constrained by the time- and cost-consuming processes needed for their morphological identification. The recent advances in high-throughput sequencing, particularly DNA metabarcoding can provide an alternative to morphology-based approaches. The main limitation for DNA-based tools to be implemented in biomonitoring projects is the considerable investment needed to build high-quality and curated taxonomic reference DNA sequence libraries for species identification. To begin addressing this shortage, GEANS project aims in developing a curated DNA reference library based on mitochondrial cytochrome c oxidase subunit I (COI) for the North Sea macrobenthos that will serve as the backbone of all the molecular protocols. meer

For the collection of the macroinvertebrates, targeted sampling campaigntake place across the North Sea Region, while existing collections are being scanned. Macrobenthic specimens are being processed following a series of standardise work flows covering sampling, morphological identifications, molecular lab processing and data handling. All specimens are photographed and together with the accompanying sequences, collection and taxonomic data are archived in a dedicate BOLD project. Voucher specimens and DNA extracts are archived at three different institutes in Germany (Senckenberg am Meer), the Netherlands (Naturalis) and Belgium (ILVO). Once available, this DNA-library will be based on taxonomically well-curated specimens and it will support the implementation of fast, cost-efficient and reliable DNA-based identifications and subsequently environmental health assessments in the North Sea.

Biologie, Biologie > Benthos, Biologie > Benthos > Macrobenthos
Marien/Kust, Brak water, Biodiversiteit, COI, Dna, DNA barcoding, Genetic, Genetic data, Marien, ANE, Noordzee, Animalia

Geografische spreiding
ANE, Noordzee [Marine Regions]

Spreiding in de tijd
1 Maart 2019 - 28 Februari 2022
Quasi continu (< 1 min.)

Taxonomic coverage
Animalia [WoRMS]

Bijdrage door
Naturalis Biodiversity Center (NBC), meerdata creator
Senckenberg am Meer, meerdata creator
Senckenberg am Meer; German Centre for Marine Biodiversity Research (DZMB), meer
Vlaamse overheid; Beleidsdomein Landbouw en Visserij; Instituut voor landbouw-, visserij en voedingsonderzoek (ILVO), meerdata creator

Gerelateerde datasets
Maakt deel uit van:
GEANS Data: Genetic tools for Ecosystem health Assessment in the North Sea region, meer

GEANS: Genetic Tools for Ecosystem Health Assessment in the North Sea Region, meer

Dataset status: Gestart
Data type: Data
Data oorsprong: Data collectie
Metadatarecord aangemaakt: 2020-03-20
Informatie laatst gewijzigd: 2022-06-15
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