Collected reprints: Abstract 2668

Collected reprints

Abstract

Campbell, R.; Adams, A.; Tatner, M.F.; Chair, M.; Sorgeloos, P. (1993). Uptake of Vibrio anguillarum vaccine by Artemia salina as a potential oral delivery system to fish fry. Fish Bulletin 3: 451-459

An ELISA to detect Vibrio anguillarum antigen in Artemia salina was developed. This assay was then used to determine the time course of antigen uptake by Artemia during incubations with Vibrio vaccine with a view to using Artemia as a vaccine carrier for oral vaccination of fish fry. The rate of uptake of vaccine by Artemia was shown to be concentration dependent. At a dose of 1.5x107 cfu ml-1, 1 mg wet weight of Artemia took up 1x105 cfu within 30min and reached maximum of 6x106 cfu after 60min. Thus each Artemia appears to have taken up approximately 1.2x105 cfu. Comparison of vaccine formulations indicated that uptake was consistently higher with formalised than with heat-treated vaccine.

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