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The NewSTHEPS MetaData catalogue gives an overview of all persons and institutes involved and lists all created datasets and publications coming out of the project.

 

Persons Institutes Publications Datasets

 

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3D FISH for the quantification of methane- and sulphur-oxidizing endosymbionts in bacteriocytes of the hydrothermal vent mussel Bathymodiolus azoricus
Halary, S.; Riou, V.; Gaill, F.; Boudier, T.; Duperron, S. (2008). 3D FISH for the quantification of methane- and sulphur-oxidizing endosymbionts in bacteriocytes of the hydrothermal vent mussel Bathymodiolus azoricus. ISME J. 2(3): 284-292. dx.doi.org/10.1038/ismej.2008.3
In: The ISME Journal: Multidisciplinary Journal of Microbial Ecology. Nature Publishing Group: London. ISSN 1751-7362; e-ISSN 1751-7370
Peer reviewed article  
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Trefwoorden
    Bathymodiolus Kenk & B. R. Wilson, 1985 [WoRMS]
    Marien/Kust
Author keywords
    3D fluorescence in situ hybridization; bacteriocyte; Bathymodiolus;methane-oxidizing bacteria; sulphur-oxidizing bacteria; symbiosis
Auteurs  Top 
  • Halary, S.
  • Riou, V.
  • Gaill, F.
  • Boudier, T.
  • Duperron, S.
Abstract
    Dual endosymbioses involving methane- and sulphur-oxidizing bacteria occur in the gills of several species of mussels from deep-sea hydrothermal vents and cold seeps. Variations of total and relative abundances of symbionts depending on local environmental parameters are not yet understood, due to a lack of reliable quantification of bacteria in the host tissue. Here, we report the first attempt to quantify volumes occupied by each type of symbiont in bacteriocyte sections from a vent mussel, Bathymodiolus azoricus, using fluorescence in situ hybridization (FISH) coupled to three dimentional microscopy and image analysis carried out by a dedicated software, which we developped. Bacteriocytes from mussels recovered at different vent sites displayed significantly different abundances of bacteria. Specimens kept in aquaria at atmospheric pressure and exposed to an artificial pulse of sulphur displayed an increase in absolute and relative abundance of sulphur oxidizers within their bacteriocytes. Distributions of all measured parameters fitted normal distributions, indicating that bacteriocytes from a specimen tend to display similar behaviours. This study shows that symbiont volume quantification is tractable using 3D FISH, and confirms the impact of local environmental parameters on symbiont abundances.
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