The tolerance of Litopenaeus vannamei larvae to increasing concentrations of total ammonia nitrogen (TAN) using a short-term static renewal method at 26°C, 34 g L-1 salinity and pH 8.5 was assessed. The median lethal concentration (24 h LC50) for TAN in zoea (1-2-3), mysis (1-2-3) and postlarvae 1 were, respectively, 4.2-9.9-16.0; 19.0-17.3-17.5 and 13.2 mg L-1TAN (0.6-1.5-2.4; 2.8-2.5-2.6 and 1.9 mg L-1NH3-N). The LC50 values obtained in this study suggest that zoeal and post-larval stages are more sensitive to 24 h ammonia exposure than the mysis stage of L. vannamei larvae. On the basis of the ammonia toxicity level (24 h LC50) at zoea 1, we recommend that this level does not exceed 0.42 mg L-1 TAN – equivalent to 0.06 mg L-1NH3-N – to reduce ammonia toxicity during the rearing of L. vannamei larvae.
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