IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Identification of cultured Brachionus rotifers based on RFLP and SSCP screening
Papakostas, S.; Dooms, S.; Christodoulou, M.; Triantafyllidis, A.; Kappas, I.; Dierckens, K.; Bossier, P.; Sorgeloos, P.; Abatzopoulos, T.J. (2007). Identification of cultured Brachionus rotifers based on RFLP and SSCP screening, in: VLIZ Coll. Rep. 35-36(2005-2006). VLIZ Collected Reprints: Marine and Coastal Research in Flanders, 35-36: pp. Chapter 59
In: (2007). VLIZ Coll. Rep. 35-36(2005-2006). VLIZ Collected Reprints: Marine and Coastal Research in Flanders, 35-36. Flanders Marine Institute (VLIZ): Oostende, more
In: VLIZ Collected Reprints: Marine and Coastal Research in Flanders. Vlaams Instituut voor de Zee: Oostende. ISSN 1376-3822, more

Also published as
  • Papakostas, S.; Dooms, S.; Christodoulou, M.; Triantafyllidis, A.; Kappas, I.; Dierckens, K.; Bossier, P.; Sorgeloos, P.; Abatzopoulos, T.J. (2006). Identification of cultured Brachionus rotifers based on RFLP and SSCP screening. Mar. Biotechnol. 8(5): 547-559. dx.doi.org/10.1007/s10126-005-6181-z, more

Available in  Authors 

Keywords
    DNA; Hatcheries; Mitochondria; Rotifera [WoRMS]; Marine
Author keywords
    COI restriction map; hatchery strains; rotifer clones; mitochondrial DNA; 16S rRNA

Authors  Top 
  • Papakostas, S.
  • Dooms, S., more
  • Christodoulou, M.
  • Triantafyllidis, A.
  • Kappas, I.
  • Dierckens, K., more
  • Bossier, P., more
  • Sorgeloos, P., more
  • Abatzopoulos, T.J.

Abstract
    The marine finfish industry worldwide depends greatly on the mass culture of Brachionus rotifers. Recently, molecular data have revealed a more complicated view about the species status of Brachionus rotifers than previous mainly morphological assessments. Under this view, Brachionus rotifers are comprised of many morphologically similar, albeit genetically differentiated, cryptic members of larger groups. A redefinition of the cultured rotifer species/biotypes is therefore needed if aquaculture is to reach higher levels of standardization and predictability. In this work, restriction fragment length polymorphism (RFLP) and single-strand conformational polymorphism (SSCP) methods are applied to the COI and 16S rRNA mitochondrial genes. A detailed COI restriction map was constructed, using sequence data from all known representatives of Brachionus phylogroups. Therefore, it is the first time that such an extended restriction database has been produced. Several restriction endonucleases are proposed for the discrimination of the different Brachionus species/biotypes. Furthermore, eight different SSCP gel alleles are described for the 16S region. Using these data, five Brachionus species/biotypes were identified in 78 samples collected from laboratories and hatcheries around the world.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors