| one publication added to basket [141563] | The route of entry and progression of infectious haematopoietic necrosis virus in Oncorhynchus mykiss (Walbaum): a sequential immunohistochemical study
Drolet, B.S.; Rohovec, J.S.; Leong, J.C. (1994). The route of entry and progression of infectious haematopoietic necrosis virus in Oncorhynchus mykiss (Walbaum): a sequential immunohistochemical study. J. Fish Dis. 17(4): 337-347
In: Journal of Fish Diseases. Blackwell Science: Oxford; London; Edinburgh; Boston; Melbourne. ISSN 0140-7775; e-ISSN 1365-2761, meer
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| Trefwoorden |
Alkaline phosphatase
Anatomical structures > Body organs > Animal organs > Respiratory organs > Gills
Anatomical structures > Circulatory system
Chemistry > Biochemistry > Histochemistry
Chemistry > Biology > Biochemistry > Enzymology > Biochemistry > Histology > Histoenzymology > Chemistry > Histology > Histochemistry > Immunochemistry > Immunohistochemistry
Diseases > Animal diseases > Fish diseases
Gastrointestinal tract
Laboratory culture > Tissue culture
Organs
Pathology
Viral diseases
Oncorhynchus mykiss (Walbaum, 1792) [WoRMS]
Marien/Kust; Brak water; Zoet water |
| Auteurs | | Top |
- Drolet, B.S.
- Rohovec, J.S.
- Leong, J.C.
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| Abstract |
Steelhead trout, Oncorhynchus mykiss (Walbaum), fry were experimentally infected with infectious haematopoietic necrosis virus (IHNV) Round Butte 1983 (Type 1). Fry were sampled daily, before and during the epizootic. Fish tissues were tested for infectious virus by tissue culture assay and for IHNV nucleocapsid protein by alkaline phosphatase immunohistochemistry (APIH). The progression of virus through the tissues was followed by APIH until the fourteenth day. Viral infection progressed from two major sites: from the gills into the circulatory system; and from the oral region into the gastrointestinal tract and then into the circulatory system. Once in the blood, virus was disseminated to virtually every organ. Progression of IHNV within and between organs is discussed. |
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