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Quantification of trypsin with a radioimmunoassay in hering larvae (Clupea harengus) compared with a highly sensitive fluorescence technique to determine tryptic enzyme activity
Ueberschär, B.; Pedersen, B.H.; Hjelmeland, K. (1992). Quantification of trypsin with a radioimmunoassay in hering larvae (Clupea harengus) compared with a highly sensitive fluorescence technique to determine tryptic enzyme activity. Mar. Biol. (Berl.) 113: 469-473
In: Marine Biology. Springer: Heidelberg; Berlin. ISSN 0025-3162, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Ueberschär, B.
  • Pedersen, B.H.
  • Hjelmeland, K.

Abstract
    Enzymatic activity and quantity of the protease trypsin were measured in individual herring larvae (Clupea harengus L.). The enzymatic activity assay was done using a fluorescence technique, and a radioimmunoassay was used for quantification of trypsin. The results are compared and the differences between the techniques discussed. Both methods gave similar results, as high or low values in trypsin quantity were reflected in high or low values of tryptic activity. Quantity and activity were linearly and positively correlated, but small differences between methods were found at the lowest detection limits. Both techniques reflect high variability between individual larvae.

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