IMIS | Flanders Marine Institute

Flanders Marine Institute

Platform for marine research


Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Flow Cytometric Applicability of Fluorescent Vitality Probes on Phytoplankton
Peperzak, L.; Brussaard, C.P.D. (2011). Flow Cytometric Applicability of Fluorescent Vitality Probes on Phytoplankton. J. Phycol. 47(3): 692-702.
In: Journal of Phycology. Blackwell Science: New York. ISSN 0022-3646, more
Peer reviewed article  

Available in  Authors 

Author keywords
    Calcein-AM; CMFDA (CellTracker Green); DiBAC(4)(3); FDA; flow cytometry;H(2)DCFDA; live/dead assay; phytoplankton; SYTOX-GREEN; vitality

Authors  Top 
  • Peperzak, L., more
  • Brussaard, C.P.D., more

    The applicability of six fluorescent probes (four esterase probes: acetoxymethyl ester of Calcein [Calcein-AM], 5-chloromethylfluorescein diacetate [CMFDA], fluorescein diacetate [FDA], and 2',7'-dichlorofluorescein diacetate [H(2)DCFDA]; and two membrane probes: bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC(4)(3)] and SYTOX-Green) as vitality stains was tested on live and killed cells of 40 phytoplankton strains in exponential and stationary growth phases, belonging to 12 classes and consisting of four cold-water, 26 temperate, and four warm-water species. The combined live/dead ratios of all six probes indicated significant differences between the 12 plankton classes (P < 0.01) and between individual species (P < 0.05). No specific differences were observed among strains of one species, among species or strains from different origin, nor between cells in exponential and stationary growth phase except for FDA. FDA showed a significant (P < 0.05) drop of <20% in fluorescence intensity in stationary cells. Of the four esterase probes, the live/dead ratios of FDA and CMFDA were not significantly different from each other, and both performed better than Calcein-AM and H(2)DCFDA (P < 0.001). Of the two membrane probes, DIBAC(4)(3) stained rhodophytes and euglenophytes much better than SYTOX-Green. The 13 algal strains best stainable (high live/dead ratios) among all six probes belonged to,nine genera from six classes of phytoplankton. In conclusion, FDA, CMFDA, DIBAC(4)(3), and SYTOX-Green represent a wide choice of vitality probes in the study of phytoplankton ecology, applicable in many species from different algal classes, originating from different regions and at different stages of growth.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors