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Freshness assessment of turbot (Scophthalmus maximus) by Quality Index Method (QIM), biochemical, and proteomic methods
Li, X.; Chen, Y.; Cai, L.; Xu, Y.; Yi, S.; Zhu, W.; Mi, H.; Li, J.; Lin, H. (2017). Freshness assessment of turbot (Scophthalmus maximus) by Quality Index Method (QIM), biochemical, and proteomic methods. Lebensm.-Wiss. Technol. 78: 172-180. https://hdl.handle.net/10.1016/j.lwt.2016.12.037
In: Lebensmittel-Wissenschaft und Technologie = Food science and technology = Science et technologie alimentaire. Elsevier: Amsterdam. ISSN 0023-6438; e-ISSN 1096-1127, more
Peer reviewed article  

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Keyword
    Scophthalmus maximus (Linnaeus, 1758) [WoRMS]
Author keywords
    Turbot; Freshness evaluation; Quality index method (QIM); Proteomic method

Authors  Top 
  • Li, X.
  • Chen, Y.
  • Cai, L.
  • Xu, Y.
  • Yi, S.
  • Zhu, W.
  • Mi, H.
  • Li, J.
  • Lin, H.

Abstract
    The freshness of turbot stored at 4 °C for 18 days was assessed using Quality Index Method (QIM), biochemical, and proteomic methods, respectively. Results showed that the developed QIM scheme for turbot consisted of eight parameters which gave a total of 20 demerit points. QI showed a linear relationship to storage time (QI = 1.175 × tdays – 0.395, R2 = 0.988), and the remaining storage time could be estimated with an accuracy of ±1 day. pH, K values, TVB-N, and TVC increased significantly with storage time, and the shelf-life of turbot was determined to have been 15 days. Seven protein spots of interest were screened form the post mortem muscle proteome during storage, and were identified as: phosphoglucomutase-1, pyruvate kinase, kinesin-1 heavy chain, Troponin T, desmin-like, and actin, respectively. They were considered as the potential freshness markers of turbot, since the abundance changes of those proteins were significantly correlated with QI, K value, TVB-N, and TVC, and associated with the loss of fish quality and freshness. The work could provide useful information enabling the development of new strategies for freshness evaluation based on protein markers.

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