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| Differences in the digestive biochemistry between the intertidal clam, Ruditapes decussatus, and the subtidal clam, Venerupis pullastra |  |
| Albentosa, M.; Moyano, F.J. (2009). Differences in the digestive biochemistry between the intertidal clam, Ruditapes decussatus, and the subtidal clam, Venerupis pullastra Aquacult. Int. 17(3): 273-282 |
| In: Aquaculture International. Chapman & Hall/Kluwer/Springer: London. ISSN 0967-6120, meer |
| Trefwoorden |
Carbohydrases; Clams; Protease; Marien |
| Abstract |
The present study was aimed to evaluate differences in physicochemical
characteristics (pH and temperature optima) of the main digestive enzymes (amylase,
cellulase, laminarinase, and protease) in two commercially important bivalves, the intertidal
clam, Ruditapes decussatus, and the subtidal clam, Venerupis pullastra, as well to
assess individual variability of such activities. The assays were performed on extracts
obtained from the digestive glands and crystalline styles of individuals collected in the wild
and laboratory-conditioned during 1 week. In both species, maximum activity for amylase
and cellulase was measured at pH 6 and that of laminarinase at pH 5.5, while pH profiles
of protease activity showed important differences between them. Temperature profiles also
showed interspecific variations, mainly in laminarinase. Carbohydrase activities were
comparatively much higher in R. decussatus and proteolytic activities in V. pullastra. The
observed differences both in physicochemical characteristics and relative importance of
digestive enzymes are discussed in relation to the amount and type of food available in
their respective habitats. |
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