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| Characterisation of neurotoxic polypeptides from Cyanea capillata medusae (Scyphozoa) |
| Lassen, S.; Helmholz, H.; Ruhnau, C.; Prange, A. (2010). Characterisation of neurotoxic polypeptides from Cyanea capillata medusae (Scyphozoa), in: Purcell, J.E. et al. (Ed.) (2010). Jellyfish blooms: New problems and solutions. Developments in Hydrobiology, 212: pp. 213-221 |
| In: Purcell, J.E.; Angel, D.L. (Ed.) (2010). Jellyfish blooms: New problems and solutions Developments in Hydrobiology, 212 Springer: Dordrecht. ISBN 978-90-481-9540-4. 234 pp., meer |
| In: Dumont, H.J. (Ed.) Developments in Hydrobiology. Kluwer Academic/Springer: Den Haag. ISSN 0167-8418, meer |
| Ook gepubliceerd als |
- Lassen, S.; Helmholz, H.; Ruhnau, C.; Prange, A. (2010). Characterisation of neurotoxic polypeptides from Cyanea capillata medusae (Scyphozoa) Hydrobiologia 645(1): 213-221, meer
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| Abstract |
Cnidarian venoms include neurotoxins, which are able to paralyse prey organisms immediately. Important targets for neurotoxins are voltage-gated ion channels in membranes of excitable cells. By blocking specific receptor sites, neurotoxic components disturb the physiological ion channel functions. Here, we describe the isolation and characterisation of potential neurotoxic polypeptides from the crude tentacle venom of the boreal scyphomedusan Cyanea capillata. Partially purified venom fractions were obtained by size-exclusion and subsequent reversed-phase chromatography. To assess the blocking activity of the venom on voltage-gated sodium channels, we modified a mouse neuroblastoma (MNB) cell assay. Venom fractions containing channel-blocking activity were analysed by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The resulting mass spectra revealed a cluster of singly charged peptides within a mass range from 3,900 to 7,000 Da. A group of three potentially neurotoxic peptides with molecular masses of 3983.4, 5795.4 and 6962.1 Da could be tracked throughout the purification process. This investigation of the crude venom is part of a multidimensional assay-guided approach for the isolation and structural characterisation of toxic polypeptides in northern Scyphozoa. |
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