DNA primers for amplification of mitochondrial cytochrome c oxidase subunit 1 from diverse metazoan invertebrates
Folmer, O.; Black, M.; Hoeh, W.; Lutz, R.; Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit 1 from diverse metazoan invertebrates. Mol. Mar. Biol. Biotech. 3(5): 294-299
In: Molecular Marine Biology and Biotechnology. Springer-Verlag: New York. ISSN 1053-6426; e-ISSN 1435-0637, more
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| Keywords |
Acids > Organic compounds > Organic acids > Nucleic acids > DNA
Aquatic organisms > Marine organisms > Aquatic animals > Marine invertebrates
Cell constituents > Cell organelles > Mitochondria
Enzymes > Coenzymes > Cytochromes
Polymerase chain reaction
Annelida [WoRMS]; Arthropoda [WoRMS]; Coelenterata [WoRMS]; Echinodermata [WoRMS]; Echiura [WoRMS]; Mollusca [WoRMS]; Vestimentifera [WoRMS]
Marine/Coastal |
| Authors | | Top |
- Folmer, O.
- Black, M.
- Hoeh, W.
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| Abstract |
We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at the species and higher taxonomic levels. |
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