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The encysted dormant embryo proteome of Artemia sinica
Zhou, Q.; Wu, C.; Dong, B.; Liu, F.; Xiang, J. (2008). The encysted dormant embryo proteome of Artemia sinica. Mar. Biotechnol. 10(4): 438-446. dx.doi.org/10.1007/s10126-007-9079-0
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228, more
Peer reviewed article  

Available in Authors 

Keywords
    Mass spectrometry; Mass spectrometry; Mass spectrometry; Artemia sinica Cai, 1989 [WoRMS]; Marine

Authors  Top 
  • Zhou, Q.
  • Wu, C.
  • Dong, B.
  • Liu, F.
  • Xiang, J.

Abstract
    The possibility of the brine shrimp Artemia to produce dormant embryo (cysts) in diapause is a key feature in its life history. In the present study, we obtained a proteomic reference map for the diapause embryo of Artemia sinica using two-dimensional gel electrophoresis with a pH range of 4-7 and a molecular weight range of 10-100 kDa. Approximately 233 proteins were detected, and 60 of them were analyzed by capillary liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these, 39 spots representing 33 unique proteins were identified, which are categorized into functional groups, including cell defense, cell structure, metabolism, protein synthesis, proteolysis, and other processes. This reference map will contribute toward understanding the state of the diapause embryo and lay the basis and serve as a useful tool for further profound studies in the proteomics of Artemia at different developmental stages and physiological conditions.

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