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Cryopreservation of sperm from spotted wolffish
Gunnarsson, S.; Sigurdsson, S.; Thorarensen, H.; Imsland, A.K. (2009). Cryopreservation of sperm from spotted wolffish. Aquacult. Int. 17(4): 385-389. dx.doi.org/10.1007/s10499-008-9210-x
In: Aquaculture International. Springer: London. ISSN 0967-6120, more
Peer reviewed article

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Keywords
    Freeze thaw durability; Freezing storage; Sperm; Anarhichas minor Olafsen, 1772 [WoRMS]; Marine

Authors  Top 
  • Gunnarsson, S.
  • Sigurdsson, S.
  • Thorarensen, H.
  • Imsland, A.K.

Abstract
    The effects of different concentrations of cryoprotectant (dimethyl sulfoxide; DMSO), cooling rate and straw size on the post-thaw motility of frozen sperm from spotted wolffish, Anarhichas minor, were studied. There was no significant difference in the post-thaw motility of sperm treated with three different concentrations of DMSO (10, 20 and 30%). Similarly, there was no significant difference in the post-thaw motility of spermatozoa when using different freezing rates (i.e. distance of straws from the surface of liquid N2, 4.7, 5.5 and 7.1°C min−1) and the straw size (0.5 and 1.0 ml) did not affect survival. The cryopreservation of sperm can be used to make up for the frequent lack of sperm and/or the unsynchronised timing of sperm production in spotted wolffish males and the ovulation time in females. The results show that sperm from spotted wolffish can be frozen to secure access to viable sperm, but further experiments are needed in order to reveal the effect of different parameters on the post-thawing mortality and define the optimum conditions for cryopreservation.

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