|Effect of temperature on a-tocopherol, fatty acid profile, and pigments of Diacronema vlkianum (Haptophyceae)|
|Durmaz, Y.; Donato, M.; Monteiro, M.; Gouveia, L.; Nunes, M.L.; Pereira, T.G.; Gökpinar, S.; Bandarra, N.M. (2009). Effect of temperature on a-tocopherol, fatty acid profile, and pigments of Diacronema vlkianum (Haptophyceae). Aquacult. Int. 17(4): 391-399. dx.doi.org/10.1007/s10499-008-9211-9|
|In: Aquaculture International. Springer: London. ISSN 0967-6120, more|
Algae; Fatty acids; Laboratory culture; Pigments; Vitamin E; Water temperature; Diacronema vlkianum Prauser, 1958 [WoRMS]; Marine; Fresh water
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Diacronema vlkianum was grown in polyethylene bags at two different temperatures (18 and 26°C) in the laboratory. The biochemical composition level decreased when the temperature increased from 18 to 26°C. The maximum cell number at 18°C was11.9 × 106 cells ml−1, while maximum cell number at 26°C was 1.6 × 106 cells ml−1. The maximum level of α-tocopherol was 257.7 ± 21.6 μg g−1 dry weight (DW) at 18°C. The highest total carotenoids and chlorophylls were 6.5 mg g−1 DW and 4.3 mg g−1 DW, respectively, and the main pigments were determined as astaxanthin and lutein. Polyunsaturated fatty acids were found to be the predominant group, reaching 39.5% of the total fatty acids at 18°C. This comprised 20:5(n − 3) as the main polyunsaturated fatty acids (20.4%, at 18°C) followed by 22:6(n − 3) (4.8%, at 18°C). The results suggest that D. vlkianum can be successfully used as feed in shellfish hatcheries or aquaculture hatcheries, either as a substitute or in association with other microalgae, when this algae is cultured at 18°C.