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Production of intestine-specific monoclonal antibody and interspecific cross-reaction in triclads and polyclads
Asano, Y.; Yoshida, A.; Isozaki, N.; Ishida, S. (2001). Production of intestine-specific monoclonal antibody and interspecific cross-reaction in triclads and polyclads. Belg. J. Zool. 131(Suppl. 1): 137-141
In: Belgian Journal of Zoology. Koninklijke Belgische Vereniging voor Dierkunde = Société royale zoologique de Belgique: Gent. ISSN 0777-6276, more
Peer reviewed article  

Also published as
  • Asano, Y.; Yoshida, A.; Isozaki, N.; Ishida, S. (2001). Production of intestine-specific monoclonal antibody and interspecific cross-reaction in triclads and polyclads, in: Saló, E. et al. (Ed.) Proceedings of the 9th International Symposium on the Biology of the Turbellaria, Barcelona, Spain, June 2000 [CD-ROM]. Belgian Journal of Zoology, 131(Suppl. 1): pp. 137-141, more

Available in  Authors 
Document type: Conference paper

Keywords
    Platyhelminthes [WoRMS]; Marine

Authors  Top 
  • Asano, Y.
  • Yoshida, A.
  • Isozaki, N.
  • Ishida, S.

Abstract
    We have produced a monoclonal antibody (mAb(2)2B6) specific to intestinal and epidermal cells of Dugesia japonica. This mAb(2)2B6 also reacted with the secretory granules of a kind of sub-epidermal gland cells, epithelial cells and gland cells in the pharynx. The results of the interspecific cross-reaction tests with four other freshwater triclads and four polyclads showed that this mAb(2)2B6 can be used as a marker, specific to only intestinal cells in both Planocera multitentaculata and Planocera reticulata of polyclads. Immuno-electron microscopical research revealed that the cytoplasm of phagocytic cells and the spherical large granules in granular clubs reacted strongly to this mAb in the intestine. Also, mAb positive material seemed to be absorbed by pinocytosis from phagocytic cells into the granular clubs. These observations support the theory that the granular clubs are protein reserve cells, not gland cells for intraluminal digestion.

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