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Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis
Chimetto, L.; Cleenwerck, I.; Brocchi, M.; Willems, A.; De Vos, P.; Thompson, F. (2011). Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis. Int. J. Syst. Evol. Microbiol. 61: 1170-1175. dx.doi.org/10.1099/ijs.0.024661-0
In: International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology: Reading. ISSN 1466-5026 , more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Chimetto, L.
  • Cleenwerck, I., more
  • Brocchi, M.
  • Willems, A., more
  • De Vos, P., more
  • Thompson, F.

Abstract
    A Gram-negative, aerobic bacterium, designated strain R-40503T, was isolated from mucus of the reef-builder coral Mussismilia hispida, located in the São Sebastião Channel, São Paulo, Brazil. Phylogenetic analyses revealed that strain R-40503T belongs to the genus Marinomonas. The 16S rRNA gene sequence similarity of R-40503T was above 97?% with the type strains of Marinomonas vaga, M. basaltis, M. communis and M. pontica, and below 97?% with type strains of the other Marinomonas species. Strain R-40503T showed less than 35?% DNA–DNA hybridization (DDH) with the type strains of the phylogenetically closest Marinomonas species, demonstrating that it should be classified into a novel species. Amplified fragment length polymorphism (AFLP), chemotaxonomic and phenotypic analyses provided further evidence for the proposal of a novel species. Concurrently, a close genomic relationship between M. basaltis and M. communis was observed. The type strains of these two species showed 78?% DDH and 63?% AFLP pattern similarity. Their phenotypic features were very similar, and their DNA G+C contents were identical (46.3 mol%). Collectively, these data demonstrate unambiguously that Marinomonas basaltis is a later heterotypic synonym of Marinomonas communis. Several phenotypic features can be used to discriminate between Marinomonas species. The novel strain R-40503T is clearly distinguishable from its neighbours. For instance, it shows oxidase and urease activity, utilizes l-asparagine and has the fatty acid C12?:?1 3-OH but lacks C10?:?0 and C12?:?0. The name Marinomonas brasilensis sp. nov. is proposed, with the type strain R-40503T (?=?R-278T ?=?LMG 25434T ?=?CAIM 1459T). The DNA G+C content of strain R-40503T is 46.5 mol%.

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