IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Developing a genetic manipulation system for the Antarctic archaeon, Halorubrum lacusprofundi: investigating acetamidase gene function
Liao, Y.; Williams, T.J.; Walsh, J.C.; Ji, M.; Poljak, A.; Curmi, P.M.G.; Duggin, I.G.; Cavicchioli, R. (2016). Developing a genetic manipulation system for the Antarctic archaeon, Halorubrum lacusprofundi: investigating acetamidase gene function. NPG Scientific Reports 6(34639): 15 pp. http://hdl.handle.net/10.1038/srep34639
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322, more
Peer reviewed article  

Available in  Authors 

Authors  Top 
  • Liao, Y.
  • Williams, T.J.
  • Walsh, J.C.
  • Ji, M.
  • Poljak, A.
  • Curmi, P.M.G.
  • Duggin, I.G.
  • Cavicchioli, R.

Abstract
    No systems have been reported for genetic manipulation of cold-adapted Archaea. Halorubrum lacusprofundi is an important member of Deep Lake, Antarctica (similar to 10% of the population), and is amendable to laboratory cultivation. Here we report the development of a shuttle-vector and targeted gene-knockout system for this species. To investigate the function of acetamidase/formamidase genes, a class of genes not experimentally studied in Archaea, the acetamidase gene, amd3, was disrupted. The wild-type grew on acetamide as a sole source of carbon and nitrogen, but the mutant did not. Acetamidase/formamidase genes were found to form three distinct clades within a broad distribution of Archaea and Bacteria. Genes were present within lineages characterized by aerobic growth in low nutrient environments (e.g. haloarchaea, Starkeya) but absent from lineages containing anaerobes or facultative anaerobes (e.g. methanogens, Epsilonproteobacteria) or parasites of animals and plants (e.g. Chlamydiae). While acetamide is not a well characterized natural substrate, the build-up of plastic pollutants in the environment provides a potential source of introduced acetamide. In view of the extent and pattern of distribution of acetamidase/formamidase sequences within Archaea and Bacteria, we speculate that acetamide from plastics may promote the selection of amd/fmd genes in an increasing number of environmental microorganisms.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors