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| Comparison of gene expression profiles of Fenneropenaeus chinensis challenged with WSSV and Vibrio |  |
| Wang, B.; Li, F.; Luan, W.; Xie, Y.; Zhang, C.; Luo, Z.; Gui, L.; Yan, H.; Xiang, J. (2008). Comparison of gene expression profiles of Fenneropenaeus chinensis challenged with WSSV and Vibrio Mar. Biotechnol. 10(6): 664-675 |
| In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228, meer |
| Trefwoorden |
Cdna array; Fenneropenaeus chinensis [WoRMS]; Vibrio [WoRMS]; Marien |
| Auteurs | | Top |
- Wang, B., publicatielijst
- Li, F., publicatielijst
- Luan, W., publicatielijst
- Xie, Y., publicatielijst
- Zhang, C., publicatielijst
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| Abstract |
Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio. |
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