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Comparative genomics of Flavobacterium columnare unveils novel insights in virulence and antimicrobial resistance mechanisms
Declercq, A.M.; Tilleman, L.; Gansemans, Y.; De Witte, C.; Haesebrouck, F.; Van Nieuwerburgh, F.; Smet, A.; Decostere, A. (2021). Comparative genomics of Flavobacterium columnare unveils novel insights in virulence and antimicrobial resistance mechanisms. Vet. Res. 52(1): 18. https://hdl.handle.net/10.1186/s13567-021-00899-w
In: Veterinary research. BIOMED CENTRAL LTD: Paris. ISSN 0928-4249; e-ISSN 1297-9716, more
Peer reviewed article  

Available in  Authors 

Keywords
    Flavobacterium columnare
    Marine; Fresh water
Author keywords
    Genome comparison, Virulence, Antimicrobial resistance

Authors  Top 
  • Declercq, A.M., more
  • Tilleman, L., more
  • Gansemans, Y., more
  • De Witte, C., more
  • Haesebrouck, F., more
  • Van Nieuwerburgh, F., more
  • Smet, A., more
  • Decostere, A., more

Abstract
    This study reports the comparative analyses of four Flavobacterium columnare isolates that have different virulence and antimicrobial resistance patterns. The main research goal was to reveal new insights into possible virulence genes by comparing the genomes of bacterial isolates that could induce tissue damage and mortality versus the genome of a non-virulent isolate. The results indicated that only the genomes of the virulent isolates possessed unique genes encoding amongst others a methyl-accepting chemotaxis protein possibly involved in the initial colonization of tissue, and several VgrG proteins engaged in interbacterial competition. Furthermore, comparisons of genes unique for the genomes of the highly virulent (HV) carp and trout isolates versus the, respectively, low and non-virulent carp and trout isolates were performed. An important part of the identified unique virulence genes of the HV-trout isolate was located in one particular gene region identified as a genomic island. This region contained araC and nodT genes, both linked to pathogenic and multidrug-resistance, and a luxR-gene, functional in bacterial cell-to-cell communication. Furthermore, the genome of the HV-trout isolate possessed unique sugar-transferases possibly important in bacterial adhesion. The second research goal was to obtain insights into the genetic basis of acquired antimicrobial resistance. Several point-mutations were discovered in gyrase-genes of an isolate showing phenotypic resistance towards first and second-generation quinolones, which were absent in isolates susceptible to quinolones. Tetracycline-resistance gene tetA was found in an isolate displaying acquired phenotypic resistance towards oxytetracycline. Although not localized on a prophage, several flanking genes were indicative of the gene’s mobile character.

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