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Cryopreservation of sterlet (Acipenser ruthenus) sperm
Horváth, Á.; Urbányi, B. (2000). Cryopreservation of sterlet (Acipenser ruthenus) sperm, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 441
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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Keyword
    Marine/Coastal
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  • Horváth, Á.
  • Urbányi, B.
Abstract
    Experiments were carried out in order to evaluate a reliable method for the cryopreservation of sterlet (Acipenser ruthenus L.) sperm. The effects of three cryoprotectants - dimethyl-sulfoxide (DMSO), dimethyl-acetamide (DMA) and methanol - and three basic extenders - two organic and one saline - were investigated on the motility and fertilising capacity of sterlet sperm. Best post-thaw motility (46 plus or minus 23%) was achieved using 10 % methanol. No significant difference was detected between the two organic extenders. The use of methanol resulted in significantly higher fertilisation rate (22 plus or minus 15%) than DMSO (2 plus or minus 4%) and no fertilisation was detected using DMA.
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