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Development and validation of a quantitative UHPLC-MS/MS method for selected brominated flame retardants in food
Malysheva, S.V.; Goscinny, S.; Malarvannan, G.; Poma, G.; Andjelkovic, M.; Voorspoels, S.; Covaci, A.; Van Loco, J. (2018). Development and validation of a quantitative UHPLC-MS/MS method for selected brominated flame retardants in food. Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment 35(2): 292-304. https://dx.doi.org/10.1080/19440049.2017.1393110
In: Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment. TAYLOR & FRANCIS LTD. ISSN 1944-0049; e-ISSN 1944-0057, more
Peer reviewed article  

Available in  Authors 

Author keywords
    Brominated flame retardants; UHPLC-MS/MS; bromophenols; HBCDsTBBPA; foodanalysis

Authors  Top 
  • Malysheva, S.V., more
  • Goscinny, S.
  • Malarvannan, G., more
  • Poma, G., more
  • Andjelkovic, M., more
  • Voorspoels, S., more
  • Covaci, A., more
  • Van Loco, J., more

Abstract
    An ultra-high performance liquid chromatography – tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated (in-house) for the quantification of selected brominated flame retardants (BFRs), including tetrabromobisphenol A (TBBPA), hexabromocyclododecanes (HBCDs), tetrabromobisphenol S (TBBPS) and bromophenols (BPs), in various food matrices. The sample preparation consisted of extraction of TBBPS with acidified acetonitrile followed by a fast dispersive solid-phase extraction (dSPE) clean-up and extraction of the other BFRs with a mixture of hexane and dichloromethane (1:1, v/v) with subsequent clean-up using acidified silica (44%, w/w). The limits of quantification of the method varied widely for the types of food matrices and the different classes of BFRs from 4 pg g−1 wet weight (ww) to 8 ng g−1 ww. For most of the analytes the apparent recovery was in the range 70–120%, and the method precision (under repeatability conditions) was below 20%. The method was successfully applied in proficiency testing exercises as well as for analysis of various food items. Only 25% of the collected food samples contained BFRs, with 4-bromophenol and α-HBCD as the only detected compounds. The contaminated foodstuffs were fish and eggs with concentrations in the range from 48 to 305 pg g−1 ww.

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