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PCR-RFLP genotyping for Japanese and Korean populations of Pacific oyster using mitochondrial DNA noncoding region
Okimoto, T.; Hara, K.; Ishihara, T.; Aranishi, F. (2008). PCR-RFLP genotyping for Japanese and Korean populations of Pacific oyster using mitochondrial DNA noncoding region. Aquat. Ecol. 42(1): 1-4. https://dx.doi.org/10.1007/s10452-006-9064-0
In: Aquatic Ecology. Springer: Dordrecht; London; Boston. ISSN 1386-2588; e-ISSN 1573-5125, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Acids > Organic compounds > Organic acids > Nucleic acids > DNA
    Cell constituents > Cell organelles > Mitochondria
    Cultures > Shellfish culture > Mollusc culture > Oyster culture
    Crassostrea gigas (Thunberg, 1793) [WoRMS]
    Marien/Kust
Author keywords
    Pacific oyster; PCR-RFLP; mitochondrial DNA; noncoding region; culturedpopulation
Auteurs  Top 
  • Okimoto, T.
  • Hara, K.
  • Ishihara, T.
  • Aranishi, F.
Abstract
    Production of Pacific oyster Crassostrea gigas in Miyagi and Hiroshima (Japan) has gradually increased, with a marked increase in imported oysters from Goseong (Korea), and then cultured oysters of the Miyagi, Hiroshima, and Goseong populations accounting for most oyster consumption in Japan. In this study, we developed a simple PCR-RFLP analysis of a mitochondrial DNA noncoding region (mtDNA-NCR) for differentiating cultured oysters of these populations. PCR amplification yielded a 818 bp fragment comprising the entire mtDNA-NCR and parts of adjacent tRNACys and tRNAAsn genes from all the specimens. By use of a wide restriction test using 30 different restriction enzymes, only a single enzyme only, AluI, produced a unique RFLP pattern enabling us to discriminate Miyagi and Hiroshima oysters from Goseong oysters. This difference is probably because of nucleotide alteration at the presumptive AluI recognizing site on position 439 of the mtDNA-NCR. Our simple, robust and cost-effective PCR-RFLP analysis is potentially useful for population genetic investigation of cultured Pacific oyster, particularly when large numbers of specimens must be analyzed.
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