In-vitro metabolism of 3,3',4,4'-tetrachlorobiphenyl in relation to ethoxyresorufin-o-deethylase activity in liver-microsomes of some wildlife species and rat
Murk, A.; Morse, D.; Boon, J.; Brouwer, A. (1994). In-vitro metabolism of 3,3',4,4'-tetrachlorobiphenyl in relation to ethoxyresorufin-o-deethylase activity in liver-microsomes of some wildlife species and rat. Eur. J. Pharmacol., Environ. Toxicol. Pharmacol. Sect. 270(2-3): 253-261. https://dx.doi.org/10.1016/0926-6917(94)90069-8
In: European Journal of Pharmacology: Environmental Toxicology and Pharmacology Section. Elsevier Science Publishers: Amsterdam; Lausanne; London; New York; Oxford; Shannon; Tokyo. ISSN 0926-6917; e-ISSN 1878-6782, meer
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| Auteurs | | Top |
- Murk, A.
- Morse, D.
- Boon, J.
- Brouwer, A.
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| Abstract |
A qualitative study was performed of the capacity of hepatic microsomes of several wildlife species to metabolize 3,3',4,4'-tetrachlorobiphenyl (TCB). Hepatic microsomes of species environmentally exposed to polychlorinated biphenyls (PCBs): harbour porpoise (Phocoena phocoena), harbour seal (Phoca vitulina), common tern (Sterna hirundo), and hepatic microsomes from species experimentally exposed to PCBs: elder duck (Somateria mollissima), rainbow trout (Salmo gairdneri), flounder (Platichthys flesus), and Wistar rat, were incubated with 14C-labelled TCB ([14C]TCB). The mammals and birds were able to metabolize TCB at a rate that correlated with their ethoxyresorufin-O-deethylase (EROD) activity. No [14C]TCB metabolism was observed in the fish, despite elevated EROD activity in the trout. HPLC analysis of diisopropylether extracts of the microsomal incubations indicated the presence of 4-OH-, 5-OH-, and 6-OH-tetrachlorobiphenyl metabolites and a yet unidentified metabolite. The ratio of the different hydroxy metabolites formed varied for the various species. |
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