Isolation of holostane-type saponins from the black sea cucumber Holothuria atra and evaluating their anti-allergic activity: in vitro and in silico study
Elkattan, A.; Matsumoto, M.; Nagata, M.; Mittraphab, Y.; Wahab, G.A.; Ashour, A.; Zaki, A.A.; Hamed, E.A.E.; Shimizu, K. (2024). Isolation of holostane-type saponins from the black sea cucumber Holothuria atra and evaluating their anti-allergic activity: in vitro and in silico study. Cytotechnology 76(6): 721-734. https://dx.doi.org/10.1007/s10616-024-00649-8
In: Cytotechnology. Springer Science+Business Media: Dordrecht. ISSN 0920-9069; e-ISSN 1573-0778, meer
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| Trefwoord |
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| Author keywords |
Holothuria atra, Holothuroidea, holostane-type saponin, ß-hexosaminidase, anti-allergy |
| Auteurs | | Top |
- Elkattan, A.
- Matsumoto, M.
- Nagata, M.
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- Mittraphab, Y.
- Wahab, G.A.
- Ashour, A.
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- Zaki, A.A.
- Hamed, E.A.E.
- Shimizu, K.
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| Abstract |
Sea cucumbers are both versatile marine organisms and an Asian marine food known to have several medicinal efects. We evaluated the anti-aller?gic potential of some major purifed holostane-type saponins from the body wall of the black sea cucum?ber, Holothuria atra. Six saponin compounds were isolated, holothurin B (1), holothurin A (2), 24-dehy?dro echinoside A (3), desholothurin A1 (4), desholo?thurin A (5), and des 24-dehydro echinoside A (6). The structures were identifed based on spectroscopic methods and by comparison with the literature. Each compound’s inhibitory activity toward the release of β-hexosaminidase was evaluated. Among the six compounds, holothurin B (1) showed the strongest inhibition of the degranulation at all tested concen?trations in a dose-dependent manner, compared to the positive control, quercetin. We also observed that holothurin B (1) was able to alleviate the infamma?tory mediators interleukin (IL)-6, IL-13, and tumor necrosis factor-alpha (TNF-α). Holothurin B (1) also inhibited the Ca2+ infux stimulated by the cal?cium ionophore A23187, by suppressing the expres?sion of inositol-1,4,5-triphosphate receptor (IP3R) mRNA. These results suggest that (i) holothurin B (1) has good anti-allergy activity without cytotoxic?ity at efective concentrations, and (ii) this compound could be a lead compound for the treatment of aller?gic diseases and associated infammation. We also performed a molecular docking study for the tested compounds to correlate their binding modes and afnity for the IP3R with the in vitro results. The results concluded that the holostane-type saponins could be used as anti-allergy agents, which may be attributed to their holostane group. |
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