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Chromosome-level genome assembly and annotation of the tropical sea cucumber Stichopus monotuberculatus
Chen, Ting, T.; Yang, Yun, Y.; Wang, Xuan, X.; Qin, Zhou, Z.; Xie, Zhenyu, Z.; Fan, Dingding, D.; Ren, Chunhua, C.; Sun, Hongyan, H.; Luo, Peng, P.; Jiang, Xiao, X.; Long, Hao, H.; Chen, Chang, C.; Pan, Wenjie, W.; E., Zixuan, Z.; Huang, Jiasheng, J.; Huang, Qianying, Q.; Xu, Jianfeng, J.; Zhang, Zepeng, Z.; Cheng, Chuhang, C.; Yu, Suzhong, S.; Wang, Yanhong, Y.; Jiang, Fajun, F.; Yan, Aifen, A.; Hu, Chaoqun, C. (2024). Chromosome-level genome assembly and annotation of the tropical sea cucumber Stichopus monotuberculatus. Scientific Data 11(1): 1245. https://dx.doi.org/10.1038/s41597-024-03985-8
In: Scientific Data. Nature Publishing Group: London. ISSN 2052-4463; e-ISSN 2052-4463, meer
Peer reviewed article  

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Abstract
    In this study, a chromosome-level genome of the tropical sea cucumber Stichopus monotuberculatus was generated by a combination of Nanopore long-read, Illumina short-read, and Hi-C sequencing technologies. The fnal assembly was 810.54Mb in length, with contig N50 and scafold N50 values of 10.15Mb and 35.36Mb, respectively. This assembly comprised 23 pseudo-chromosomes, covering 99.82% of the genome. Completeness analysis using BUSCO indicated that 97.8% of the metazoan conserved genes were presented in their entirety. A total of 29,596 protein-coding genes were predicted, with functional annotations available for 94.43% of these genes. The high-quality genome assembly produced in this study may provide an essential foundation for future researches on resource conservation and genetic breeding of S. monotuberculatus.

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